Sli15INCENP Dephosphorylation Prevents Mitotic Checkpoint Reengagement Due to Loss of Tension at Anaphase Onset
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چکیده
منابع مشابه
Sli15INCENP Dephosphorylation Prevents Mitotic Checkpoint Reengagement Due to Loss of Tension at Anaphase Onset
The mitotic checkpoint, also known as the spindle assembly checkpoint, delays anaphase onset until all chromosomes have reached bipolar tension on the mitotic spindle [1-3]. Once this is achieved, the protease separase is activated to cleave the chromosomal cohesin complex, thereby triggering anaphase. Cohesin cleavage releases tension between sister chromatids, but why the mitotic checkpoint n...
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The cyclical phosphorylation and dephosphorylation of the centrosome during mitosis was analyzed by immunofluorescence methods using the MPM-2 antibody, which reacts with a subset of mitotic phosphoproteins. Quantification of MPMreactivity indicated that centrosomal phosphorylation attained a maximal level just prior to anaphase onset. This level was maintained in metaphase cells blocked from f...
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The mitotic checkpoint monitors the attachment of kinetochores to microtubules and delays anaphase onset until all sister kinetochores have become attached to opposite poles [1, 2]. Correct bipolar attachment leads to kinetochore deformation and tension and satisfies the checkpoint [3-6]. What prevents mitotic checkpoint reactivation when sister centromeres are split and tension is lost at anap...
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Two closely connected mechanisms safeguard the fidelity of chromosome segregation in eukaryotic cells. The mitotic checkpoint monitors the attachment of kinetochores to microtubules and delays anaphase onset until all sister kinetochores have become attached to opposite poles. In addition, an error correction mechanism destabilizes erroneous attachments that do not lead to tension at sister kin...
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ژورنال
عنوان ژورنال: Current Biology
سال: 2010
ISSN: 0960-9822
DOI: 10.1016/j.cub.2010.06.023